TUNEL Apoptosis Detection Kit (YSFluor™) _ 40307ESDuring apoptosis, cells activate certain DNA endonucleases that cleave genomic DNA between nucleosomes. When DNA is extracted from apoptotic cells and analyzed by electrophoresis, a characteristic DNA ladder of ~180200 bp fragments can be observed. The TUNEL (TdT mediated dUTP Nick End Labeling) Apoptosis Detection Kit (YSFluor 488) is designed to detect DNA fragmentation in the nuclei of cells during late stage apoptosis. The principle is based on

a difference of less than 1 degree is acceptable

Late apoptotic and necrotic cells are Annexin V⁺/PI⁺ (double-positive)

Published 2021 Aug 23

as it can dissolve in water and thus has less reaction toxicity (Fluorescein is a low-molecular-weight organic compound composed of phenylthiazole and thiazole carboxylic acid groups

Instrument Compatibility

② Increase the reaction temperature of IVT to 50-56℃

20350_Manual_HB230116_EN

The strength index of agarose is greater than or equal to 1200

RNA enzyme protection analysis

After reaching room temperature

such as in vitro reporter gene assays

Subcutaneous Tumorigenesis(Cat#40183ES)