PBS 10X with NP-40Sodium phosphate (10mM), potassium phosphate (20 mM), KCL (100 mM), NaCl (137 mM), and NP 40 (1%), pH 7. 4 + 0. 2. Prepared in 3D water and was filtered using 0. 22 micron filter. Shipped at RT, store at RT.

Lysozyme derived from hen egg whites has a higher heat tolerance than other sources

sterilize any aqueous solutions via filtration before use in cell culture

Cellulase is an enzyme used to isolate intact cells and protoplasts from plant tissue

5 ml microcentrifuge tube

or DMF first

Coat the DNA/RNA on the Gene Bullet -T and you are ready for delivery into host

If using for cell culture

N' tetraacetic acid] is of Molecular Biology grade

nucleic acid agarose electrophoresis

cost-effective forceps are 10' long and have a variety of general uses in the lab and are most suitable for plant tissue culture applications

easy-to-use Protoplast Isolation Kit is available for purchase

For a full 1X concentration